Application

Research Sub CategoryCytokines & Cytokine Receptors

Detect Migration Inhibitory Factor Related Proteins 8 & 14 using this Anti-Migration Inhibitory Factor Related Proteins 8 & 14 Antibody, clone AHN-17 validated for use in WB & IC.

Research CategoryInflammation & Immunology

Immunocytochemistry on ethanol fixed cells

Immunoblotting on un-reduced gels

Optimal dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein S100-A8/9 (UniProt: P05109/ P06702; also known as MRP-8/MRP-14, Leukocyte L1 complex light chain/ Leukocyte L1 complex heavy chain, p8/p14, S100 calcium-binding protein A8/A9) are encoded by the S100A8/S100A9 (also known as MRP8/MRP14) genes (Gene ID: 6279/6280) in human. MRP-8 and MRP-14 are calcium- and zinc-binding protein that play a prominent role in the regulation of inflammatory processes and immune response. They are known to induce neutrophil chemotaxis and adhesion. They are predominantly found as calprotectin and perform a variety of intra- and extracellular functions. Their intracellular functions include facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes, and activation of the neutrophilic NADPH-oxidase. They are known to activate NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane. MRP-8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. Their extracellular functions include proinflammatory, antimicrobial, oxidant-scavenging and induction of apoptosis. MRP-14 is reported to induce neutrophil chemotaxis and adhesion and can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 pathways and can induce degranulation of neutrophils by a MAPK-dependent mechanism. MRP-8 and MRP-14 also acts as danger associated molecular pattern (DAMP) molecules and stimulate innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation end (AGER) products. They also play an impotent role as oxidant scavengers and have a protective role in preventing exaggerated tissue damage by scavenging oxidants. (Ref.: Atallah, M., et al. (2012). PloS One. 7(2); e29333; Nakatani, Y., et al. (2005). Mediators Inflamm. 2005(5); 280-292; Ryckman, C., et al. (2003). J. Immunol. 170(6); 3233-3242).

Immunogen

Neutrophils

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: CBL269

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Format: Purified

Specificity

Migration Inhibitory Factor Releasing Proteins -8 and -14

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.